Moles Could Halt Project

[Guest]

It has emerged that a new setback hit Margate FCs return to Hartsdown Park.Nature lovers have discovered a rare breed of mole has moved into the undergrowth at the over grown Margate ground .If this is proved to be correct ,which is to be comfirmed by the WWF(World Wildlife Federation)work will not go ahead for the forseeable future as this is a protected species

[hartgeman]

what were they doing inside the ground,its still a building site and dont the club have the keys to the ground.

[hartgeman]

just check the internet and as far is i can see there are no rare breeds of moles.(what is the point of these rummers, i am getting board with these rummers, why do it do you have nothing beyter to do in you lives)

[knighty101]

lol, try and take no notice m8.

tho would be cool if the WWF aka WWE were coming to margate then we could see some ringside action <img src="/forum/images/graemlins/smile.gif" alt="" /> <img src="/forum/images/graemlins/yay.gif" alt="" />

[Guest]

i think that you will find rareist variety of mole is the CRYPTATES so seeing as i am alot more qualified in these matters please do not querie me

[knighty101]

isnt that somthing to do with biochemestry?

[hartgeman]

I know but its an un nerving time.

but i agree if wwe came to margate we could enter sandy in to a match againt the undertaker

(as sandy is sort of like him)(can you guess how)

[Guest]

yes it is shared by the formulae of because of the greyish red clour on the back like the smoke formed from the substance of what you would get if you Compared it to TBP Eu3+, a shift of the maximum of the excitation wavelength from 307 nm to 325 nm,

[hartgeman]

do you mean this:

method for the nonradioactive detection of specific amplified target DNA, based on a new long-lived fluorescent label, europium(III) trisbipyridine cryptate [TBP-(Eu3+)]. After a polymerase chain reaction with a set of nested primers, microtiter affinity-collected hybrids were recognized by a TBP(Eu3+)-labeled antibody. The particular structure of the label containing the energy transfer units (bipyridine moieties) enables direct fluorescence measurement on the solid phase. Moreover, its stability and long lifetime contribute to the enhancement of the signal-to-noise ratio. The usefulness of this new label is illustrated by the detection of human papillomavirus type 16 DNA in clinical smears.

[knighty101]

epp to many numbers <img src="/forum/images/graemlins/smile.gif" alt="" />

[hartgeman]

or this:We describe experimentally accessible diagnostics for the excitation of optically dense frequency-selective media by linear frequency-chirped pulses using a sensitive pump-probe technique on the 7F0 to 5D0 transitions of 1.0% Eu3+:Y2SiO5. Distinct features within a transmitted cw probe pulse are used to identify the combination of linear chirp rate and optical power needed to produce an average Bloch-vector rotation of 90°. The resulting superposition state is thus an equal mixture of the ground and excited states on average. We find experimentally a linear relationship between the applied chirp-pulse intensity and chirp rate required to produce this half-inversion, a conclusion supported by both analytical calculations made using a Landau-Zener approach, and detailed computer simulations using the Maxwell-Bloch model. The numerical simulations predict experimentally observed phenomena such as the reshaping of probe pulses by stimulated emission or absorption. Finally, we quantify the relationship between chirp rate and optical power for half-inversion as a function of the optical density of the medium. The pump-probe experimental techniques and simulation analysis techniques developed here can be extended to produce an arbitrary mixture of ground and excited states, on average, in media spanning a wide range of optical density. Preparation of such media by chirped pulses for applications in quantum computing, photon-echo-based time-domain storage, and signal processing will be aided by these techniques

[hartgeman]

info about

 

The selection of new fluorescent tracers and the subsequent generation of rare earth cryptates were driven by the specific constraints of homogeneous assays listed before.

Cryptates are formed by the inclusion of a cation into a tridimensional cavity. The properties of the trisbypyridine macrocycle, for instance (figure 2), favor such a tight association of the cage with the Europium ion that this interaction becomes virtually unchallegeable, and leads to an exceptionally inert complex.

 

Figure 2 : Eu3+ trisbypirydine cryptate.

In Eu3+ trisbipyridine cryptate (TBP Eu3+), the rigidity given by the bipyridine moieties tends to increase the activation parameters and a G* of approximately 25-30 kcal/mole can be reached (figure 3). These kinetically stable cryptates can be used under drastic chemical conditions, such as solid phase peptidic synthesis using Fmoc chemistry and reverse phase chromatography in the presence of trifluoroacetic acid. On the contrary, the transition state of chelates is easily achieved with G* of 0-10 kcal/mole, for example by partial protonation of the carboxylate groups. This explains why chelates, unlike cryptates, are not stable in acidic media and prone to exchange their rare earth ions with ions present in the media, like Mn2+.

 

Figure 3 : Cryptate versus chelate stability.

Thermodynamic data show that transition state of chelates ( is more attainable than that of cryptates (A) which implies cryptates are least likely to undergo dissociation.

 

 

As described earlier for chelates, the cage acts as a light collecting device and relays the energy to the core of the cryptate, the Eu3+. The long-lived emission obtained upon excitation (300 µsec to 1 msec) is characteristic of Eu3+ fluorescence between 580 and 720 nm.

Heterobifunctional reagents are used to conjugate cryptate diamine derivatives to antigens, antibodies, proteins, peptides and oligonucleotides. The photophysical properties of the photoactive cryptate are preserved in the conjugates27-28. Unlike most other rare earth chelates, cryptates possess, within the same molecule, the properties required for its use in homogeneous bioassays : light harvesting moiety, defined stoechiometry and high kinetic stability in biological media.

Concerning the protection of the Eu3+ ion against non radiative deactivation by water molecules or quenchers, photophysical studies have shown the existence of 2 to 3 free Europium co-ordination sites in TBP Eu3+ 29. Incorporation of fluoride ions in the Eu3+ co-ordination sphere allows a total shielding through the formation of ion pairs30.

 

Figure 4 : A) trisbypirydine pentacarboxylate Eu3+ cryptate and

pyridine bipyridine tetracarboxylate Eu3+ cryptate

 

 

The trisbypirydine cage has also been the basis for substitution studies, in the perspective of the production of more fluorescent cryptates. Compared to TBP Eu3+, a shift of the maximum of the excitation wavelength from 307 nm to 325 nm, more adapted for nitrogen laser excitation at 337 nm, was obtained by substituting carboxylic groups on two of the bipyridine units, leading to a 2.5 fold increase of the cryptate fluorescence (Figure 4A)31. This cryptate has been successfully introduced for assays that require a high sensitivity32-33.

Also, as quenching processes leading to the deactivation of Eu3+ excited states may be based on red-ox events leading to the transitory formation of Eu2+ ion, the size of the cryptate cavity could be decreased in order to destabilize Eu2+ formation. Indeed, pyridine bipyridine tetracarboxylate Eu3+ structure, the recently synthesized cryptate, shows a dramatic decrease in quenching processes (Figure 4B)31,34.

[hartgeman]

a little about them or it:

Rare earth cryptates and homogeneous fluoroimmunoassays with human sera

G Mathis

Cis Bio International, Laboratoire des Produits pour l'Analyse Medicale, Bagnols sur Ceze, France.

 

The rare earth cryptates are used as long-lived fluorescent labels. They are formed by inclusion of a europium ion in the intramolecular cavity of a macropolycyclic ligand containing bipyridine groups as light absorbers. The use of fluoride ions in the measuring medium allows a total shielding of the label fluorescence. Here, I have established the conditions under which the signal of these cryptates can be amplified through the use of nonradiative energy transfer, and applied these conditions to homogeneous immunoassays by using allophycocyanin as the acceptor. Because only a low proportion of the cryptate label is involved in the transfer amplification, and because allophycocyanin emission occurs between the europium emission lines, time-resolved measurement of europium and allophycocyanin emission allows real-time correction of the optical properties of the assay medium. These features have allowed the development of a rapid homogeneous immunoassay of prolactin that can detect as little as 0.3 microgram/L (3rd International Standard 84/500).

[hartgeman]

basicaly the story isnt true

[Guest]

yes but as i say it shares the name but it a type of mole

[hartgeman]

the only mole it has any thing to do with mole which stands the moleleculer size

[hartgeman]

mole means molecule not as in creature

[Hornet]

Another unregistered nob. Although I did see Sandy at Hatrtsdown Park with a spade and a box of little furry creatures in the middle of the night. He said it was a last ditch effort to f* ck things up-erm..,I mean I'm digging a ditch for perimiter fencing 'cos I want to help-I really want to see football return to Margate.And the little furry creatures he said were just fluffy bunnies for the asylum seekers of Thanet,because he really cares about the people here and after all it is Christmas. Seemed genuine to me.

 

WELL WHERE'S OUR [****!!****] CHRISTMAS PRESENT THEN?!!

[hartgeman]

very good hornet

[Hornet]

Wot a mug!! He's taken me right in. I can see now-this lot will try ANYTHING.

Agree with Legend that the WWF coming to Margate would be good publicity ( have'nt seen wrestling in ages ).

But I think the original poster of this thread says it all-T.I.S. This is sh*t. <img src="/forum/images/graemlins/wurm.gif" alt="" />